Mathematical Biology Seminar Janneke Cruts, Erasmus MC, Tuesday, Sep. 10, 2024 2:00pm in LCB 323 In vitro and in silico modeling of blood clots Abstract: Thrombosis, the formation of blood clots, can lead to serious clinical events such as ischemic stroke and myocardial infarction. In this seminar, I will provide an overview of my research aimed at improving our understanding of thrombosis using in vitro experiments and computational modeling. Initially, we investigated clot composition, mechanical properties, and clot CT characteristics using in vitro human blood clot analogues, demonstrating that CT-based predictions could help tailor endovascular treatments for ischemic stroke patients. This work is now being extended through clinical studies involving ex vivo patient clots. The focus of our recent work lies in developing in silico models of blood coagulation. We introduced a reduced 0D model with patient-specific parameters derived from thrombin generation data, which we subsequently integrated into a 1D model validated against in vitro thrombodynamics assays. This was further expanded to a 3D model simulating clot formation under flow conditions in a blood vessel. Ongoing work involves validating these models with additional patient plasma samples and an in vitro microfluidic setup. Finally, I will discuss the incorporation of platelet surface interactions into these models, a key aspect of my current project as a visiting scholar at the University of Utah.